The purpose of this study was to understand acculturation and race/ethnicity influences in the home and school environment that affect physical activity and nutrition in female adolescents attending middle school. A convenience sample of eight female adolescents (n = 2 Asian American, n = 2 Black, n =2 Latinx/Hispanic, and n = 2 White) was interviewed individually via Zoom. Responses regarding acculturation, physical activity, and nutrition in the home and school environment were analyzed using qualitative case study analysis. Three themes were identified: 1) experiences related to home, health, and culture, 2) the intersection between school meals and personal culture, and 3) the intersection between school physical activity and personal culture. Study findings can inform policies on school nutrition and physical activity and lead to opportunities for students and families to collaborate with schools to improve adolescent health.