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The Hispanic population is the largest and fastest growing population in the United States, which necessitates research on the acculturation process, especially because of the current events regarding the Texas–Mexico border. Although research has indicated the need to develop coping skills to support positive mental health during acculturation, there is a lack of empirical information regarding acculturation and mental health among Mexican alumni of U.S. colleges and universities. Based on social identity theory and acculturation theory, this quantitative, correlational study was conducted to examine the relationship between acculturation and psychological well-being (PWB) among Mexican alumni of colleges and universities in the United States. Forty-seven participant surveys were obtained via an online survey on acculturation, as measured by the Acculturation Rating Scale for Mexican Americans-II, and PWB, as measured by the Ryff Scales of Psychological Well-Being. An ANCOVA was implemented with a hierarchical multiple regression with gender and amount of time spent at the U.S. college or university as covariates. However, the results were statistically nonsignificant regarding relationships between levels of acculturation and PWB, gender, and number of years at the college or university. Thus, the results promote positive social change by encouraging the development of updated instruments and inclusion of additional demographic information in the research of Mexican alumni.